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What color is Alexa Fluor 568?

What color is Alexa Fluor 568?

orange-fluorescent

What Alexa 488?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

What is TRITC used for?

Tetramethylrhodamine (TRITC) dashboard Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.

Are FITC and GFP the same?

Available Options. Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.

What is a fluorochrome and how is it used?

Fluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength.

What does Alexa Fluor do?

Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules.

What color is Alexa Fluor 594?

Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines.

Is Cy5 far-red?

Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.

What does Alexa Fluor 568?

Invitrogen Alexa Fluor 568 dye is a bright, orange-fluorescent dye with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range.

What color is Tritc?

bright orange-fluorescent

Does Cy5 do Photobleach?

The significant solvent isotope effects observed in water with high Cy5 concentration, and in chloroform, demonstrate that singlet oxygen can contribute to fluorophore photobleaching under certain conditions. In

What Colour is Alexa Fluor 555?

yellow

What color is Alexa Fluor 647?

far-red

Is FITC same as 488?

High-performance alternatives to fluorescein The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.

Is Alexa 488 Green?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line.

What wavelength is TRITC?

TRITC (Tetramethylrhodamine-isothiocyanate) is a fluorescent compound with an excitation peak at 544 nm and an emission peak at 570 nm. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™.

Is Alexa Fluor 488 the same as FITC?

Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. However, Alexa Flour 488 display higher photostability and is less susceptible to self-quenching.

What color is Alexa Fluor 633?

-red dye

Is 488 a FITC?

Generally Alexa Fluor 488 has a brighter apple-green fluorescence compared to FITC.2015-01-20

What color is Alexa Fluor?

red

Alexa Fluor 546 dye | Thermo Fisher Scientific – US

Invitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

Alexa Fluor 546 | C44H46Cl3N4NaO14S3 – PubChem

Alexa Fluor 546 | C44H46Cl3N4NaO14S3 – PubChem compound Summary Alexa Fluor 546 Contents 1 Structures 2 Names and Identifiers 3 Chemical and Physical Properties 4 Related Records 5 Literature 6 Patents 7 Classification 8 Information Sources 1 Structures 1.1 2D Structure Find Similar Structures Chemical Structure Depiction 1.2 3D Status

SNAP-Surface® Alexa Fluor® 546 – NEB

SNAP-Surface ® Alexa Fluor ® 546 is a photostable fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells. This is a cell impermeable substrate based on Alexa Fluor 546 It is suitable for standard TAMRA or Cy3 filter sets

Alexa Fluor™ 546 Tyramide Reagent – Thermo Fisher

Alexa Fluor™ 546 Tyramide Reagent (556/573 ex/em) can be detected using a standard Orange/RFP/TRIC filter cube. Features of the SuperBoost reagents include: • Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging • Simple protocol and detection using standard filters

Alexa Fluor™ 546 Phalloidin – Thermo Fisher Scientific

Alexa Fluor™ 546 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, orange-fluorescent Alexa Fluor™ 546 dye. • Selectively stains F-actin • Outstanding fluorescence performance • Excitation/Emission: 556/570 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples

Alexa Fluor 546 dye | Thermo Fisher Scientific – DE

Invitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

Alexa Fluor® 546 Maleimide | TriLink BioTechnologies

Alexa Fluor ® 546 Maleimide – (O-0336) –> Skip to the beginning of the images gallery . This modification is available as part of a custom oligonucleotide configuration. Please use OLIGObuilder ™ to

Spectra Viewer – Alexa Fluor 546™ | Chroma Technology Corp

Alexa Fluor 350™ 442nm: 346nm: Alexa Fluor 405: 422nm: 401nm: Alexa Fluor 488™ 520nm: 498nm: Alexa Fluor 514™ 539nm: 517nm: Alexa Fluor 532™ 554nm: 531nm: Alexa Fluor 546™ 573nm: 556nm: Alexa Fluor 555™ 568nm: 553nm: Alexa Fluor 568™ 603nm: 578nm: Alexa Fluor 594™ 617nm: 590nm: Alexa Fluor 633™ 647nm: 632nm: Alexa Fluor 647

Alexa Fluor Dyes—Across the Spectrum | Thermo Fisher

With the wide range of Invitrogen Alexa Fluor dyes there is something for every filter and many options for multiplex detection. This series of superior fluorophores: Spans the near-UV, visible and near-IR spectrum ( Figure 1, Table 1 ). Are compatible with all common excitation sources ( Table 2) and instruments

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PDF Alexa Fluor® 546 Protein Labeling Kit

Alexa Fluor® 546 Protein Labeling Kit | 3 1.3 To 0.5 mL of the 2 mg/mL protein solution, add 50 µL of 1 M bicarbonate (prepared in step 1.1). Note:Bicarbonate, pH ~8.3, is added to raise the pH of the reaction mixture, since succinimidyl esters react efficiently at pH 7.5-8.5. 1.4 Allow a vial of reactive dye to warm to room temperature.

SNAP-Surface® Alexa Fluor® 546 – NEB

SNAP-Surface ® Alexa Fluor ® 546 is a photostable fluorescent substrate that can be used to label SNAP-tag ® fusion proteins in solution or on the surface of living cells. This is a cell impermeable substrate based on Alexa Fluor 546 It is suitable for standard TAMRA or Cy3 filter sets

Invitrogen Alexa Fluor 546 Phalloidin 300 units:Cell

Phalloidin binds F-actin with high selectivity while Alexa Fluor 546 provides orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor 546 phalloidin allows high-contrast discrimination of actin staining. Selectively stains F-actin Outstanding fluorescence performance

Alexa 546 Fluorescent Dye, Cy3 Alternative Dye

Bio-Synthesis offers Alexa 546 fluorescent dye oligonucleotide labeling services. Alexa 546 is a yellow -fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. This dye is pH insensitive over a wide molar range which ensure stable signal generation in imaging and flow cytometry.

Alexa Fluor 546 – an overview | ScienceDirect Topics

Alexa Fluor 488 is excited by blue light and fluoresces green; excitation and emission are in the same ranges as fluorescein (FITC). All fluorescent microscopes are equipped with this filter. For orange or red color, Alexa Fluor 546 or 594 (corresponding to rhodamine or Texas Red) can be used, based on the filter available in the microscope. •

Invitrogen Dextran, Alexa Fluor 546; 10,000 MW, Anionic

Invitrogen™ Dextran, Alexa Fluor™ 546; 10,000 MW, Anionic, Fixable. Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors J Neurochem. 2010 Aug;114(4):994-1006. doi: 10.1111/j.1471-4159.2010.06819.x. Epub 2010 May 18. Authors Arik J Hone 1

Alexa Fluor® 488-F(ab')2 Goat Anti-Human IgG, F(ab')2 (min

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for

AZDye 546 NHS Ester | Alexa Fluor 546 NHS Ester

AZDye™ 546 NHS Ester (Alexa Fluor® 546 NHS Ester) is a bright, water-soluble, orange-fluorescence emitting dye used for labeling proteins and antibodies.

Mouse IgG1 Isotype Control [Alexa Fluor® 546] (DDXCM01A546

Mouse IgG1 Isotype Control [Alexa Fluor® 546]. Available in 3 dyes & fluorophores. Backed by our 100% Guarantee.

Alexa Fluor – Wikipedia

The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name.Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and

Invitrogen™ Alexa Fluor™ 546 Phalloidin from Thermo Fisher

Alexa Fluor 546 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. It offers numerous advantages using antibody-based detection, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding.

IgG (H+L) Cross-Adsorbed Goat anti-Mouse, Alexa Fluor 546

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

IgG (H+L) Highly Cross-Adsorbed Goat anti-Rabbit, Alexa

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

Goat anti-Mouse IgG (H+L) Cross-Adsorbed, Alexa Fluor™ 546

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

Alexa Fluor® 488-F(ab')2 Goat Anti-Human IgG, Fcγ (min X

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for

Alexa546 vs Alexa555? – ResearchGate

I use 555, not 546. No big change, but I recommend to use 555 if you use 488 or GFP simultaneously. “Alexa Fluor® 488 dye conjugate showed a 15-30% better signal-to-noise ratio than the

Alexa Fluor® 488-F(ab')2 Donkey Anti-Chicken IgY (IgG) (H

Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for

PDF TABLE OF FLUOROCHROMES – Iowa State University

Alexa Fluor® 350 347 442 Alexa Fluor® 430 434 540 Alexa Fluor® 488 495 519 Alexa Fluor® 532 531 554 Alexa Fluor® 546 556 573 Alexa Fluor® 555 556 573 Alexa Fluor® 568 579 604 Alexa Fluor® 594 591 618 Alexa Fluor® 633 632 647 Alexa Fluor® 647 650 668 Alexa Fluor® 660 663 690 Alexa Fluor® 680 679 702

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PDF Fluorochromes Brightness Chart – BU

Fluor 546 Alexa Fluor 647 EX . 565 556 Filter 660120 575125 610’20 585115 670130 LP 640 550 600 e (cm-‘M-‘) 1960000 240000 116000 239000 Quantum Yield 0.68 0.90 Brightness Brightness intensity 3420000 1120000 163200 104400 78870 655 575 660 603 573 668

Alexa Fluor® conjugated secondary antibodies – Abcam

The Alexa Fluor ® dyes have been conjugated to a large number of secondary antibodies raised in different species including donkey, goat, and rabbit. A large number within the range is pre-adsorbed ensuring low species cross-reactivity – thus ideal for multi-color analysis.

Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa

Thermo Fisher Scientific’s Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 546 is a Goat Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, and Immunofluorescence. The Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor 546 Antibody was generated using IgG as the antigen and it reacts with Mouse.

Alexa Fluor® 488-F(ab')2 Goat Anti-Mouse IgG, F(ab')2 (min

Alexa Fluor® 488. Amax: 493 Emax: 519nm. Alexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures

XFD546 tyramide reagent *Same Structure to Alexa Fluor

AF546 tyramide contains the bright Alexa Fluor® 546 that can be readily detected with the standard TRITC filter set Cat No. 11075 Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.

Alexa Fluor 546-ArIB[V11L;V16A]is a potent ligand for

Alexa Fluor 546-ArIB[V11L;V16A] labels HEK293 cells expressing α7 nAChRs. Alexa Fluor 546-ArIB[V11L;V16A] is a relatively hydrophobic ligand, a property that can produce undesired non-specific binding. Additionally the peptide could bind to unknown sites on nAChRs which would not have been detected in the functional or radioligand binding assays.

iFluor™ 546 Styramide *Superior Replacement for Alexa

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. iFluor™ 546 Styramide is a superior replacement for Alexa Fluor 546 tyramide or other spectrally similar fluorescent tyramide

Alexa Fluor™ 546 Phalloidin – Thermo Fisher

Alexa Fluor™ 546 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, orange-fluorescent Alexa Fluor™ 546 dye. Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ‘death cap’ mushroom and is commonly used in imaging applications to selectively label F-actin.

Mouse IgG1 Isotype Control [Alexa Fluor® 546]

Mouse IgG1 Isotype Control [Alexa Fluor® 546]. Available in 3 dyes & fluorophores. Backed by our 100% Guarantee.

PDF Alexa Fluor ® Dyes

Alexa Fluor 488 dye and orange-fl uorescent Alexa Fluor 546 dye, the Alexa Fluor 532 dye and its conjugates are ideal for use with 532 nm excitation sources, including the frequency-doubled Nd:YAG laser. Alexa Fluor 546 Dye—A More Fluorescent Alternative to Cy3 Dye and Tetramethylrhodamine

SNAP-SURFACE® Alexa Fluor 546 , New England Biolabs | VWR

SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cellsProtein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry

PDF Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.

Alexa Fluor – an overview | ScienceDirect Topics

Alexa Fluor 546-labeled tubulin, Alexa Fluor 546 (Succinimidyl Ester, Life Technologies A-20002) 3. TAMRA-labeled tubulin, TAMRA (Succinimidyl Ester, Life Technologies C1171) 4. Axonemes (Supporting protocol 2 in Waterman-Storer, 2001) 5. PIPES (Sigma, P6757) 6. Paclitaxel (Sigma, T7191) 7. GTP (Jena Biosciences #NU-1012) 8. GMPCPP (Jena

Spectra Viewer – Alexa Fluor 546™ | Chroma Technology Corp

Chroma Spectra Viewer. Given the severity of the COVID-19 outbreak, Chroma’s top priority remains the health and well-being of our employees, customers and greater communities.

(A-11010) Goat anti-Rabbit IgG (H+L) Cross-Adsorbed

Alexa Fluor 546 Target Rabbit View Product On Supplier’s Website Request a Quote from Invitrogen Antibodies. Add to Procurement List Product is on your procurement list. View List. View more details on the supplier’s website. Supplier suggested. Applications. Flow cytometry/Cell sorting (FC/FACS)

Human IgG4 Isotype Control [Alexa Fluor® 546]

Human IgG4 Isotype Control [Alexa Fluor® 546]. Available in 6 dyes & fluorophores. Backed by our 100% Guarantee.

Alexa Fluor® 546-FluoroNanogold™ – Universal Biologicals

Alexa Fluor 546 – FluoroNanogold IgG goat anti mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (7401) Starting at: £305.28. 1.4 nm Nanogold® PLUS fluorescent dye: Secondary antibody and streptavidin conjugates Label for multiple microscopes in one step! For confocal, EM, LM, blotting and diagnostics. More Info Add to Basket.

PDF Alexa Fluor * 546 Fluoronanogold™** – Nanoprobes

Alexa Fluor® 546 FluoroNanogold™ is a unique, dual-purpose probe developed specially for confocal and electron microscopy.1 Alexa Fluor® FluoroNanogold™ reagents consist of affinity-purified Fab’ fragments conjugated to both Alexa Fluor® 546 dye and the

Alexa Fluor 546 | C44H46Cl3N4NaO14S3 | ChemSpider

Structure, properties, spectra, suppliers and links for: Alexa Fluor 546.

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Fluorophores modification | IDT – Integrated DNA Technologies

Dye 5′ Int 3′ Ex. Em. Alternative; Alexa Fluor® 488 (NHS Ester) 492: 517: ATTO™ 488 (NHS Ester) Alexa Fluor® 532 (NHS Ester) 527: 553: N/A: Alexa Fluor® 546 (NHS Ester)

Alexa Fluor 546 FluoroNanogold -Streptavidin: Instructions

Alexa Fluor® 546 FluoroNanogold™ is a unique, dual-purpose probe developed specially for confocal and electron microscopy. 1 Alexa Fluor®546 FluoroNanogold™-Streptavidin consists of streptavidin conjugated to both Alexa Fluor® 546 dye and the 1.4 nm Nanogold® particle . 2 A streptavidin molecule labeled with Alexa Fluor® 546 and

Alexa Fluor® Secondary Antibodies – Jackson Immuno

GFAPalpha DAPI primary astrocyte culture 1. Primary mixed glial culture derived from P0-P3 mouse pups, stained for GFAP C-term (Santa Cruz sc-6170) and donkey-anti-goat Alexa Fluor® 488 (Jackson ImmunoResearch 705-546-147).Authors: Carlyn Martina-Mamber, Elly M. Hol.

PDF Alexa Fluor * 546 Fluoronanogold™** – Nanoprobes

Alexa Fluor® 546 FluoroNanogold™ is a unique, dual-purpose probe developed specially for confocal and electron microscopy.1 Alexa Fluor®546 FluoroNanogold™-Streptavidin consists of streptavidin conjugated to both Alexa Fluor® 546 dye and the 1.4 nm

PDF A Guide to Fluorochromes – Abcam

HiLyte FluorTM 488 501 527 Alexa Fluor®514 518 540 EviTag TM quantum dots-Catskill Green 525 540 Alexa Fluor®430 434 541 Pacific Orange™ 403 551 Alexa Fluor®532 532 554 HEX 535 556 EviTag TM quantum dots-Hops Yellow 545 560 Chromeo TM 546 545 561 Cy3® 548 561 488,514 Alexa Fluor®555 555 565 HiLyte FluorTM 555 550 566 5-TAMRA 541 568

Alexa Fluor 546 Phalloidin | Thermo Fisher | Bioz

Alexa Fluor 546 phalloidin was used to stain F-actin in macrophages. Maximum intensity projections obtained by merging 11 sections from the base of the coverslip (~ 4 μm from the base into the cell) are shown. Representative projected images for control macrophages are shown in panel ( A ) and the corresponding images for macrophages treated

Goat Anti Rabbit Alexa Fluor 546 Igg | Thermo Fisher | Bioz

Thermo Fisher alexa fluor 546 conjugated goat anti rabbit igg Alexa Fluor 546 Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS – scores, article reviews, protocol conditions and more

Alexa Fluor 488 anti-human HIF1alpha Antibody anti-HIF1a

HeLa cells were treated overnight (top) or untreated (bottom) with CoCl 2, then intracellularly stained with HIF1α (clone 546-16) Alexa Fluor® 488 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).

Have you ever used Alexa Fluor 488 and 594 as a

My lab also has kinds of Alexa Fluor conjugated secondary antibodies, like 488, 546, probably from Invitrogen (I’ll check it later). Under the scanning condition for Alexa Fluor 488

Immune regulation by fibroblasts in tissue injury depends

(B and C) Uptake of Alexa Fluor 647-labeled murine MBL-A (white) by dermal fibroblasts in vivo was assessed using confocal imaging 24 h after subcutaneous injection into col1a1.GFP mice. MBL was coinjected with either Alexa Fluor 546-labeled 10 kD dextran (B, red) or Alexa Fluor 555-labeled anti-uPARAP mAb 2h9 (C, red).

Multi Band Filter Sets – Multi Band Filter Sets | Chroma

Given the severity of the COVID-19 outbreak, Chroma’s top priority remains the health and well-being of our employees, customers and greater communities.

Alexa Fluor 546 Conjugated Donkey Anti Mouse Igg – Bioz

Thermo Fisher alexa fluor 546 conjugated donkey anti mouse igg Alexa Fluor 546 Conjugated Donkey Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS – scores, article reviews, protocol conditions and more

An Introduction to Alexa Dyes – Bitesize Bio

Alexa conjugated samples have excellent signal amplification and are usually diluted in blocking buffer to reduce non-specific binding. For instance, a concentration of as low as 1-10 ?g/mL Alexa Fluor IgG conjugate can be used for fluorophore- and biotin-labeled antibodies and tandem-labeled antibodies in immunohistochemical applications.

Donkey Anti Mouse Igg Conjugated To Alexa Fluor 546

Article Snippet: Alexa Fluor 546-conjugated anti-mouse (A10036), anti-rabbit (A21085) IgG, and Alexa Fluor 647-labeled donkey anti-mouse IgG (A21236) were purchased from Invitrogen (Carlsbad, CA, USA).

Alexa Fluor 546 Goat Anti-Mouse IgG (H+L) a-11003 from

Item Alexa Fluor 546 Goat Anti-Mouse IgG (H+L) Company Thermo Fisher Scientific; Catalog Number a-11003 This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists.

PILR-alpha Antibody (36H2) [Alexa Fluor® 546] – Novus Bio

View Rat Monoclonal anti-PILR-alpha Antibody (36H2) [Alexa Fluor® 546] (DDX0230A546-100). Validated Applications: Flow, ICC/IF. Validated Species: Human.

Rat IgG3 Isotype Control [Alexa Fluor® 546] – Novus Bio

Rat IgG3 Isotype Control [Alexa Fluor® 546]. Available in 3 dyes & fluorophores. Backed by our 100% Guarantee.

Goat Anti Rabbit (IgG) secondary antibody Alexa Fluor® 488

The secondary antibody (green) was ab150077 Alexa Fluor ® 488 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor 680 dye | Thermo Fisher Scientific – CN

Alexa Fluor 680 dye. Invitrogen Alexa Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range. The fluorescence quantum yield and high photostability in antifade allow

121 questions with answers in ALEXA FLUOR | Science topic

Recently my lab has run into an issue when using Alexa Fluor 647 on mouse brain tissue (PFA fixed/frozen IHC). When viewed under an epifluorescence microscope (Y5 filter cube: Excitation: 590-650

Used Resourses: